Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage M1

I K Toth; V Mulholland; V Cooper; S Bentley; Y-L Shih; M C M Perombelon; G P C Salmond

doi:10.1099/00221287-143-7-2433

Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage M1

Microbiology (Reading). 1997 Jul;143(7):2433-2438. doi: 10.1099/00221287-143-7-2433.

Authors

I K Toth^{1

2}, V Mulholland², V Cooper², S Bentley², Y-L Shih², M C M Perombelon¹, G P C Salmond³

Affiliations

¹ Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK.
² Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK.
³ Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK.

PMID: 33657724
DOI: 10.1099/00221287-143-7-2433

Abstract

Using enrichment methods, a new bacteriophage (M1) was isolated, which is capable of generalized transduction in Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043. M1 is probably a virulent phage and contains double-stranded DNA of approximately 43 kb. Transduction frequencies for a number of chromosomal markers and plasmid pHCP2 were established, and conditions for transduction optimized. UV irradiation of the lysates prior to transduction enhanced the transduction frequency. M1 infected over 25% of Eca strains tested and so may be useful both for the genetic analysis of a number of Eca isolates and for the transductional transfer of selectable markers between strains.

Keywords: Erwinia carotovora subsp; atroseptica; bacteriophage. M1; generalized transduction; potato blackleg.