Production and Purification of Dengue Virus-like Particles from COS-1 Cells

Jedhan Ucat Galula; Gwong-Jen J Chang; Day-Yu Chao

doi:10.21769/BioProtoc.3280

Production and Purification of Dengue Virus-like Particles from COS-1 Cells

Bio Protoc. 2019 Jun 20;9(12):e3280. doi: 10.21769/BioProtoc.3280.

Authors

Jedhan Ucat Galula¹, Gwong-Jen J Chang², Day-Yu Chao¹

Affiliations

¹ Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.
² Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, US Department of Health and Human Services, Fort Collins, Colorado, USA.

Abstract

Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires abundant, and high-purity production methods. Here, we describe a robust protocol for producing DENV VLPs from transiently-transformed or stable COS-1 cells and further provide an easily adaptable antigen purification method by sucrose gradient centrifugation.

Keywords: COS-1 cell; Dengue virus (DENV); Sucrose gradient centrifugation; VLP purification; Virus-like particle (VLP).