Association of Polymorphism in Survivin Gene and the Risk of Liver Cancer Resulting from Hepatitis C Virus Among Egyptian Patients

Amal A Mohamed; Aymen S Yassin; Basma S Gomaa; Hossam Darwish; Rasha S Mohamed; Sahar Makled; Ahmed Ramdan; Sherief Abd-Elsalam; Marwa M Raafat

doi:10.2174/1568009621666210302090917

Association of Polymorphism in Survivin Gene and the Risk of Liver Cancer Resulting from Hepatitis C Virus Among Egyptian Patients

Curr Cancer Drug Targets. 2021;21(6):536-543. doi: 10.2174/1568009621666210302090917.

Authors

Amal A Mohamed¹, Aymen S Yassin², Basma S Gomaa³, Hossam Darwish⁴, Rasha S Mohamed⁵, Sahar Makled⁶, Ahmed Ramdan⁷, Sherief Abd-Elsalam⁸, Marwa M Raafat³

Affiliations

¹ Biochemistry Department, National Hepatology and Tropical Medicine Research Institution, Cairo, Egypt.
² Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
³ Microbiology and Immunology Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, Cairo, Egypt.
⁴ Oncology Department, Ismailia Teaching Hospital, Ismailia, Egypt.
⁵ Department of Internal Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.
⁶ Tropical Department, National Hepatology and Tropical Medicine Research Institute Cairo, Egypt.
⁷ Department of Tropical, Faculty of Medicine, Cairo University, Cairo, Egypt.
⁸ Department of Tropical Medicine and Infectious Diseases, Faculty of Medicine, Tanta University, Tanta, Egypt.

PMID: 33653251
DOI: 10.2174/1568009621666210302090917

Abstract

Background: This study aims to investigate the relation between Survivin gene polymorphisms and the risk of Hepatocellular carcinoma (HCC) resulting from hepatitis C infection among the Egyptian population.

Methods: This prospective study was conducted on 164 patients, 57 patients were diagnosed with hepatitis C, where 57 were diagnosed with HCC in addition to 50 healthy volunteers as controls. Genotyping for Survivin rs1042489 and rs8073069 single nucleotide polymorphisms was carried out by the allelic discrimination Real-Time Polymerase Chain Reaction Single Nucleotide Polymorphisms genotyping technology.

Results: The results of Survivin rs1042489 polymorphism revealed that the TC and CC genotypes were significantly different between hepatocellular carcinoma patients (OR=15.5, 95%CI: 3.299-72.825,P<0.001), and controls (OR=44, 95%CI: 8.025-241.254, P<0.001). Furthermore, CC genotype was significantly different between cirrhotic and hepatocellular carcinoma patients (OR=19.2, 95%CI: 3.097-119.049, P=0.002). Moreover, the TC genotype shows a significant difference between controls and cirrhotic patients (OR=5.5, 95%CI: 2.111-14.328, P<0.001). However, when comparing TT genotypes, CC+TC genotypes results showed a significant association with increasing the risk of cirrhosis and hepatocellular carcinoma (OR=4.812, 95%CI: 1.893-12.233, P=0.001), (OR=21.607, 95%CI: 4.738-98.532, P<0.01), respectively. On the other hand, there was no significant difference among all studied groups for all genotypes regarding Survivin rs8073069. Also, the CC+GC genotype showed no significant association with increased risk of hepatocellular carcinoma (P=0.999) compared with the GG genotypes.

Conclusion: The study indicates that functional Survivin rs1042489 polymorphism may contribute to the risk of hepatocellular carcinoma while Survivin rs8073069 polymorphism has no significant association with increased risk of hepatocellular carcinoma among the studied groups.

Keywords: BIRC5; Egypt; HCC; HCV; RT-PCR.; SNP; Survivin; apoptosis; gene.

MeSH terms

Carcinoma, Hepatocellular* / genetics
Case-Control Studies
Egypt
Genetic Predisposition to Disease
Hepacivirus
Hepatitis C*
Humans
Liver Neoplasms* / genetics
Polymorphism, Single Nucleotide
Prospective Studies
Survivin / genetics

Substances

Survivin