In Vitro Assessment of Prebiotic Activity

Alberto Amaretti; Stefano Raimondi; Nicola Volpi; Maddalena Rossi

doi:10.1007/978-1-0716-1274-3_17

In Vitro Assessment of Prebiotic Activity

Methods Mol Biol. 2021:2278:209-223. doi: 10.1007/978-1-0716-1274-3_17.

Authors

Alberto Amaretti^{1

2}, Stefano Raimondi¹, Nicola Volpi¹, Maddalena Rossi^{3

4}

Affiliations

¹ Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
² BIOGEST-SITEIA, University of Modena and Reggio Emilia, Modena, Italy.
³ Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy. maddalena.rossi@unimore.it.
⁴ BIOGEST-SITEIA, University of Modena and Reggio Emilia, Modena, Italy. maddalena.rossi@unimore.it.

PMID: 33649959
DOI: 10.1007/978-1-0716-1274-3_17

Abstract

Bifidogenic effect is a main target for the assessment of prebiotic activity. pH-controlled batch processes of bifidobacteria and fecal microbiota are herein presented. Growth of bifidobacteria, carbohydrate breakdown and consumption, organic acid production, and activity of specific glycosyl hydrolases involved in the hydrolysis of di-, oligo-, or polysaccharides are exploited to study and compare substrate preference of bifidobacteria for candidate prebiotics.

Keywords: Batch; Bifidobacterium; Bioreactor; Glycosyl-hydrolase; HPAEC-PAD; HPTLC-AMD; Microbiota cultures; Prebiotic.

MeSH terms

Bacterial Proteins / metabolism
Batch Cell Culture Techniques / methods
Bifidobacterium / chemistry
Bifidobacterium / growth & development
Bifidobacterium / metabolism*
Bioreactors
Carbohydrate Metabolism
Chromatography, High Pressure Liquid / methods
Chromatography, Ion Exchange / methods
Chromatography, Thin Layer / methods
Enzyme Assays / methods
Fermentation
Gastrointestinal Microbiome
Humans
Hydrolases / metabolism
Hydrolysis
Polysaccharides, Bacterial / analysis
Polysaccharides, Bacterial / metabolism*

Substances

Bacterial Proteins
Polysaccharides, Bacterial
Hydrolases