Phageome Analysis of Bifidobacteria-Rich Samples

Methods Mol Biol. 2021:2278:71-85. doi: 10.1007/978-1-0716-1274-3_7.

Abstract

Bifidobacteria are important early colonizers of the human intestinal tract. The relative abundance of bifidobacterial species may be modulated, in part, by bacteriophage activity. Metagenomic studies of these populations is a crucial step in understanding this important interaction. This chapter outlines the technical instructions required to analyze the virome of a bifidobacteria-rich sample, for example, an infant fecal sample.

Keywords: DNA; Fecal; Metagenome; Phage; Sequencing.

MeSH terms

  • Bacteriophages / genetics*
  • Bifidobacterium / virology*
  • DNA, Viral / genetics
  • Feces / microbiology*
  • Gastrointestinal Microbiome
  • High-Throughput Nucleotide Sequencing / methods
  • Humans
  • Infant
  • Virome*

Substances

  • DNA, Viral