Quantitative PCR for Tenacibaculum dicentrarchi and T. finnmarkense

J Fish Dis. 2021 May;44(5):655-659. doi: 10.1111/jfd.13357. Epub 2021 Mar 1.

Abstract

Numerous Tenacibaculum species, including T. dicentrarchi, T. maritimum and T. finnmarkense, are associated with tenacibaculosis in finfish; however, quantitative identification techniques are limited. Quantitative PCR assays were developed to detect T. dicentrarchi and T. finnmarkense. TaqMan assays using 16S rDNA demonstrated low detection limits (0.07-269 bacteria), suitable amplification efficiencies (>86%) and moderate specificity. However, the amplification of isolates with 100% sequence similarity to T. finnmarkense AY7486TD using both the T. finnmarkense and T. dicentrarchi assays indicates that other genes should be investigated. Both assays may help describe the pathogenesis of tenacibaculosis and may aid management practices for the aquaculture industry.

Keywords: TaqMan; Tenacibaculum dicentrarchi; Tenacibaculum finnmarkense; qPCR; tenacibaculosis.

MeSH terms

  • Animals
  • Fish Diseases / diagnosis*
  • Flavobacteriaceae Infections / diagnosis
  • Flavobacteriaceae Infections / veterinary*
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / veterinary*
  • Tenacibaculum / isolation & purification*

Supplementary concepts

  • Tenacibaculum dicentrarchi
  • Tenacibaculum finnmarkense