The Physiological Response of Lettuce to Red and Blue Light Dynamics Over Different Photoperiods

Giedrė Samuolienė; Akvilė Viršilė; Jurga Miliauskienė; Perttu J Haimi; Kristina Laužikė; Aušra Brazaitytė; Pavelas Duchovskis

doi:10.3389/fpls.2020.610174

The Physiological Response of Lettuce to Red and Blue Light Dynamics Over Different Photoperiods

Front Plant Sci. 2021 Feb 12:11:610174. doi: 10.3389/fpls.2020.610174. eCollection 2020.

Authors

Giedrė Samuolienė¹, Akvilė Viršilė¹, Jurga Miliauskienė¹, Perttu J Haimi¹, Kristina Laužikė¹, Aušra Brazaitytė¹, Pavelas Duchovskis¹

Affiliation

¹ Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, Babtai, Lithuania.

Abstract

This study aimed to evaluate the effect of dynamic red and blue light parameters on the physiological responses and key metabolites in lettuce and also the subsequent impact of varying light spectra on nutritive value. We explored the metabolic changes in carotenes, xanthophylls, soluble sugars, organic acids, and antioxidants; the response of photosynthetic indices [photosynthetic (Pr) and transpiration (Tr) rates]; and the intracellular to ambient CO₂ concentration ratios (C _i /C _a ) in lettuce (Lactuca sativa L. "Lobjoits Green Cos"). They were cultivated under constant (con) or parabolic (dyn) blue (B, 452 nm) and/or red (R, 662 nm) light-emitting diode (LED) photosynthetic photon flux densities (PPFDs) at 12, 16, and 20 h photoperiods, maintaining consistent daily light integrals (DLIs) for each light component in all treatments, at 2.3 and 9.2 mol m^-2 per day for blue and red light, respectively. The obtained results and principal component analysis (PCA) confirmed a significant impact of the light spectrum, photoperiod, and parabolic profiles of PPFD on the physiological response of lettuce. The 16 h photoperiod resulted in significantly higher content of xanthophylls (neoxanthin, violaxanthin, lutein, and zeaxanthin) in lettuce leaves under both constant and parabolic blue light treatments (BconRdyn 16 h and BdynRdyn 16 h, respectively). Lower PPFD levels under a 20 h photoperiod (BdynRdyn 20 h) as well as higher PPFD levels under a 12 h photoperiod (BdynRdyn 12 h) had a pronounced impact on leaf gas exchange indices (Pr, Tr, C _i /C _a ), xanthophylls, soluble sugar contents, and antioxidant properties of lettuce leaves. The parabolic PPFD lighting profile over a 16 h photoperiod (BdynRdyn 16 h) led to a significant decrease in C _i /C _a , which resulted in decreased Pr and Tr, compared with constant blue or red light treatments with the same photoperiod (BconRdyn and BdynRcon 16 h). Additionally, constant blue lighting produced higher α + β-carotene and anthocyanin (ARI) content and increased carotenoid to chlorophyll ratio (CRI) but decreased biomass accumulation and antioxidant activity.

Keywords: antioxidant activity; carotenes; dynamic light; organic acids; photoperiod; photosynthesis; soluble sugars; xanthophylls.