One-step synthesis of multifunctional nanoparticles for CT/PA imaging guided breast cancer photothermal therapy

Liwen Fu; Shuguang Yang; Shichao Jiang; Xiaojun Zhou; Zhou Sha; Chuanglong He

doi:10.1016/j.colsurfb.2021.111630

One-step synthesis of multifunctional nanoparticles for CT/PA imaging guided breast cancer photothermal therapy

Colloids Surf B Biointerfaces. 2021 May:201:111630. doi: 10.1016/j.colsurfb.2021.111630. Epub 2021 Feb 18.

Authors

Liwen Fu¹, Shuguang Yang¹, Shichao Jiang², Xiaojun Zhou¹, Zhou Sha¹, Chuanglong He³

Affiliations

¹ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.
² Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, China.
³ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China. Electronic address: hcl@dhu.edu.cn.

PMID: 33639508
DOI: 10.1016/j.colsurfb.2021.111630

Abstract

Advances in nanotheranostics have promoted the development of precision medicine, which has great potential as a weapon for clinical diagnosis and therapy of tumors. However, the combination of three functional principle components (imaging probes, therapeutic agents and surface coating) in traditional theranostic system is difficult to be achieved in only one step, while undergoing multiple synthesis procedures, time-consuming process and unknown toxicity. Herein, we fabricated iodinated polyaniline (LC@I-PANi) nanoparticles via a facile one-step synthesis approach integrating chemical oxidative polymerization and iodine-doping process for computed tomography (CT) imaging and photoacoustic (PA) imaging-guided photothermal therapy (PTT). Iodic acid (HIO₃) as an oxidant induces chemical oxidation polymerization of aniline monomers. Meanwhile, iodine is incorporated into the polyaniline structural units in the process of polymerization to obtain LC@I-PANi nanoparticles. Moreover, thel-cysteine (LC) has an effect on diameter of LC@I-PANi nanoparticles, which enables nanoparticles have size-controlled spherical morphology and good colloidal stability. The hemolysis assay and cytotoxicity assessment verified the good biocompatibility of LC@I-PANi. Moreover, our LC@I-PANi nanoparticles could not only exhibit appealing PTT efficiency, but also achieve excellent CT/PA dual-mode imaging effect. The histological evaluations suggested the negligible toxicity of LC@I-PANi in vivo. This is the first time to our knowledge that multifunctional LC@I-PANi nanoparticles were prepared by an ingenious one-step method. This work not only highlights a one-step strategy that simplified the complex synthesis of LC@I-PANi nanoparticles, but also provides insight for further biomedical application of "all-in-one" theranostic agent.

Keywords: Dual-mode imaging; Iodinated polyaniline; Nanotheranostics; One-step synthesis; Photothermal therapy.

MeSH terms

Breast Neoplasms* / diagnostic imaging
Breast Neoplasms* / drug therapy
Female
Humans
Multifunctional Nanoparticles*
Nanoparticles*
Phototherapy
Photothermal Therapy
Theranostic Nanomedicine
Tomography, X-Ray Computed