Prevention of necrosis caused by transient expression in Nicotiana benthamiana by application of ascorbic acid

Shohei Nosaki; Mika K Kaneko; Fuminori Tsuruta; Hideki Yoshida; Yukinari Kato; Kenji Miura

doi:10.1093/plphys/kiab102

Prevention of necrosis caused by transient expression in Nicotiana benthamiana by application of ascorbic acid

Plant Physiol. 2021 Jun 11;186(2):832-835. doi: 10.1093/plphys/kiab102.

Authors

Shohei Nosaki^{1

2}, Mika K Kaneko³, Fuminori Tsuruta¹, Hideki Yoshida^{1

2}, Yukinari Kato^{3

4}, Kenji Miura^{1

2}

Affiliations

¹ Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan.
² Tsukuba-Plant Innovation Research Center, University of Tsukuba, Tsukuba 305-8572, Japan.
³ Graduate School of Medicine, Tohoku University, Sendai 980-8575, Japan.
⁴ New Industry Creation Hatchery Center, Tohoku University, Sendai 980-8575, Japan.

Abstract

Application of high concentrations of sodium ascorbate suppresses necrosis caused by the expression of recombinant proteins in Nicotiana benthamiana, resulting in an increase in protein accumulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies / metabolism
Ascorbic Acid / administration & dosage*
Cullin Proteins / genetics
F-Box-WD Repeat-Containing Protein 7 / genetics
Gene Expression
Humans
Necrosis / prevention & control*
Nicotiana / drug effects*
Nicotiana / genetics
Plant Diseases / prevention & control*
Plant Leaves / drug effects
Plant Leaves / genetics
Plants, Genetically Modified
Recombinant Proteins
Transgenes

Substances

Antibodies
Cullin 1
Cullin Proteins
F-Box-WD Repeat-Containing Protein 7
FBXW7 protein, human
Recombinant Proteins
Ascorbic Acid