Metal-responsive reversible binding of triplex-forming oligonucleotides with 5-hydroxyuracil nucleobases

Kotaro Nishiyama; Keita Mori; Yusuke Takezawa; Mitsuhiko Shionoya

doi:10.1039/d1cc00553g

Metal-responsive reversible binding of triplex-forming oligonucleotides with 5-hydroxyuracil nucleobases

Chem Commun (Camb). 2021 Mar 9;57(20):2487-2490. doi: 10.1039/d1cc00553g.

Authors

Kotaro Nishiyama¹, Keita Mori¹, Yusuke Takezawa¹, Mitsuhiko Shionoya¹

Affiliation

¹ Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. shionoya@chem.s.u-tokyo.ac.jp.

PMID: 33616595
DOI: 10.1039/d1cc00553g

Abstract

Metal-responsive triplex-forming oligonucleotides (TFOs) were synthesised by incorporating 5-hydroxyuracil (UOH) nucleobases as metal recognition sites. Binding of the UOH-containing TFO to the target natural DNA duplexes was reversibly regulated by the addition and removal of GdIII ions under isothermal conditions.

MeSH terms

Base Sequence
Cations / chemistry
DNA / chemistry*
Gadolinium / chemistry
Hydrogen Bonding
Nucleic Acid Conformation
Oligonucleotides / chemistry*
Phase Transition
Uracil / analogs & derivatives*
Uracil / chemistry

Substances

Cations
Oligonucleotides
triplex DNA
5-hydroxyuracil
Uracil
DNA
Gadolinium