Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies

Ji-Hong Bong; Tae-Hun Kim; Jaeyong Jung; Soo Jeong Lee; Jeong Soo Sung; Chang Kyu Lee; Min-Jung Kang; Hyun Ok Kim; Jae-Chul Pyun

doi:10.1007/s13206-021-00011-6

Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies

Biochip J. 2021;15(1):100-108. doi: 10.1007/s13206-021-00011-6. Epub 2021 Feb 17.

Authors

Ji-Hong Bong¹, Tae-Hun Kim¹, Jaeyong Jung¹, Soo Jeong Lee¹, Jeong Soo Sung¹, Chang Kyu Lee¹, Min-Jung Kang², Hyun Ok Kim³, Jae-Chul Pyun¹

Affiliations

¹ Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722 Republic of Korea.
² Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), 5 Hwarang-ro 14-gil, Seongbuk-gu, Seoul, 02792 Republic of Korea.
³ Department of Laboratory Medicine, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722 Republic of Korea.

Abstract

Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.

Keywords: Anti-SARS-CoV-2 nucleoprotein antibody; Competitive assay; Culture fluid; Nucleoprotein (NP); SARS-CoV-2.