Fungal community succession contributes to product maturity during the co-composting of chicken manure and crop residues

Abstract

The succession of the fungal community during the co-composting of chicken manure and crop residues and its role in relation to compost maturity was deciphered using Illumina sequencing and FUNGuild (Fungi + Functional + Guild) tool. In the maturation phase of composting, the relative abundance of pathogenic and symbiotrophic fungi decreased by 68%-85% and 145%-622%, respectively, as compared to the initial phase, which showed 574%-720% increase in the saprotrophic guild. The pathogenic and saprotrophic fungi abundance was correlated to compost maturity represented by germination index and humic spectroscopic ratio (p < 0.05). Random forest analysis and structural equation modeling elucidated the positive effects of the aforementioned fungal taxa on compost maturity, and these effects were mediated by the micro-environmental variables, such as temperature, NH₄⁺-N/NO₃^--N ratio and total organic carbon content. Our study outlines the importance of fungal community succession for improving composting performance and efficiency.

Keywords: Compost maturity; Composting; FUNGuild; Functional succession; Fungal community.