A Fucoxanthinol Induces Apoptosis in a Pancreatic Intraepithelial Neoplasia Cell

Masaru Terasaki; Takuya Inoue; Wataru Murase; Atsuhito Kubota; Hiroyuki Kojima; Mareshige Kojoma; Tohru Ohta; Hayato Maeda; Kazuo Miyashita; Michihiro Mutoh; Mami Takahashi

doi:10.21873/cgp.20248

A Fucoxanthinol Induces Apoptosis in a Pancreatic Intraepithelial Neoplasia Cell

Cancer Genomics Proteomics. 2021 Mar-Apr;18(2):133-146. doi: 10.21873/cgp.20248.

Authors

Masaru Terasaki^{1

2}, Takuya Inoue³, Wataru Murase³, Atsuhito Kubota³, Hiroyuki Kojima^{3

2}, Mareshige Kojoma³, Tohru Ohta², Hayato Maeda⁴, Kazuo Miyashita⁵, Michihiro Mutoh⁶, Mami Takahashi⁷

Affiliations

¹ School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan; terasaki@hoku-iryo-u.ac.jp.
² Advanced Research Promotion Center, Health Sciences University of Hokkaido, Hokkaido, Japan.
³ School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan.
⁴ Faculty of Agriculture and Life Science, Hirosaki University, Aomori, Japan.
⁵ Center for Industry-University Collaboration, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
⁶ Department of Molecular-Targeting Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
⁷ Central Animal Division, National Cancer Center, Tokyo, Japan.

Abstract

Background/aim: Fucoxanthinol (FxOH), a predominant metabolite from fucoxanthin (Fx), can exert potential anti-cancer effects in various cancers. However, limited data are available on the effect of FxOH or Fx on pancreatic cancer. The present study investigated the effect of FxOH on a cell line derived from pancreatic cancer tissue developed in Ptf1a^Cre/+; LSL-k-ras^G12D/+ mice.

Materials and methods: Using flow-cytometric, microarrays, and western blotting analyses, alterations in FxOH-induced apoptosis-related gene expression and protein levels were evaluated in a mice pancreatic cancer cell line, KMPC44.

Results: FxOH significantly arrested the cells at S phase along with suppression of many gene sets, such as cytokine- cytokine receptor interaction and cell adhesion molecule CAMS. Moreover, attenuated protein levels for cytokine receptors, adhesion, phosphatidylinositol-3 kinase/protein kinase B, and mitogen-activated protein kinase were observed.

Conclusion: FxOH may prevent pancreatic cancer development in a murine cancer model.

Keywords: Apoptosis; NCAM2; carotenoid; fucoxanthinol; mouse pancreatic cancer.

Publication types

Review

MeSH terms

Animals
Apoptosis
Carcinoma in Situ / drug therapy*
Disease Models, Animal
Female
Humans
Mice
Pancreatic Neoplasms / drug therapy*
beta Carotene / analogs & derivatives
beta Carotene / pharmacology

Substances

beta Carotene
fucoxanthinol