A sensitive sandwich ELISA using a modified biotin-streptavidin amplified system for histamine detection in fish, prawn and crab

Food Chem. 2021 Jul 15:350:129196. doi: 10.1016/j.foodchem.2021.129196. Epub 2021 Feb 5.

Abstract

Histamine poisoning from seafood is a significant public health and safety concern. To detect histamine sensitively and accurately, a novel competitive sandwich immunoassay using a modified biotin-streptavidin system coupling with polylysine was developed. Using this strategy, a sandwich ELISA with an IC50 value of 112.8 ng mL-1 and a broad linear range of 11.7-1500 ng mL-1 with a correlation coefficient of 0.9942 was validated. Without any sample derivatization procedure, the recovery of histamine ranged from 80.19% to 108.3% with a coefficient of variation of 1.43-11.7% in tuna, prawn and crab. The sandwich ELISA had a detectionlimit of 5.86 ng mL-1, which was 15-fold lower than an indirect competitive ELISA (ic-ELISA). This simple, sensitive and accurate method can be applied to detect histamine in routine seafood samples.

Keywords: Biotin-streptavidin; Histamine; Sandwich ELISA; Seafood samples; ic-ELISA.

MeSH terms

  • Animals
  • Biotin / metabolism*
  • Brachyura / chemistry*
  • Decapoda / chemistry*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Food Analysis
  • Histamine / analysis*
  • Limit of Detection
  • Seafood / analysis
  • Streptavidin / metabolism*
  • Tuna*

Substances

  • Biotin
  • Histamine
  • Streptavidin