Tethered Function Assay to Study RNA-Regulatory Proteins in Zebrafish Embryos

Yuichiro Mishima; Kunio Inoue

doi:10.1007/978-1-0716-0970-5_27

Tethered Function Assay to Study RNA-Regulatory Proteins in Zebrafish Embryos

Methods Mol Biol. 2021:2218:347-354. doi: 10.1007/978-1-0716-0970-5_27.

Authors

Yuichiro Mishima¹, Kunio Inoue²

Affiliations

¹ Department of Frontier Life Sciences, Faculty of Lifesciences, Kyoto Sangyo University, Kyoto, Japan. mishima@cc.kyoto-su.ac.jp.
² Department of Biology, Graduate School of Science, Kobe University, Kobe, Hyogo, Japan.

PMID: 33606244
DOI: 10.1007/978-1-0716-0970-5_27

Abstract

Many proteins are assumed to mediate post-transcriptional regulation of mRNAs. However, the lack of information about their target mRNAs and functional domains hampers the detailed analysis of their molecular function. Here we describe a method to analyze the post-transcriptional effects of proteins of interest by artificially tethering the protein to a reporter mRNA in zebrafish embryos.

Keywords: Post-transcriptional regulation; Tethering; Translation; Zebrafish; mRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biological Assay / methods*
Embryo, Nonmammalian / physiology*
RNA / genetics*
RNA Processing, Post-Transcriptional / genetics
RNA, Messenger / genetics
RNA-Binding Proteins / genetics*
Zebrafish / genetics*
Zebrafish Proteins / genetics*

Substances

RNA, Messenger
RNA-Binding Proteins
Zebrafish Proteins
RNA