Pseudorandom full-field electroretinograms reflect different light adaptation mechanisms

Abstract

Purpose: To investigate the magnitude and time course of pseudorandom ffERG during light adaptation.

Methods: Ten healthy subjects (26 ± 10.1 years) underwent 20 min of dark adaptation, and then the ffERG was evoked by pseudorandom flash sequences (4 ms per flash, 3 cd.s/m²) driven by m-sequences (2¹⁰-1 stimulus steps) using Veris Science software and a Ganzfeld dome over a constant field of light adaptation (30 cd/m²). The base period of the m-sequence was 50 ms. Each stimulation sequence lasting 40 s was repeated at 0, 5, 10, 15 and 20 min of light adaptation. Relative amplitude and latency (corrected by values found at 0 min) of the three components (N1, P1, and N2) of first-order (K₁) and first slice of the second-order (K_2.1) kernel at 5 time points were evaluated. An exponential model was fitted to the mean amplitude and latency data as a function of the light adaptation duration to estimate the time course (τ) of the light adaptation for each component. Repeated one-way ANOVA followed by Tukey post-test was applied to the amplitude and latency data, considering significant values of p < 0.05.

Results: Regarding the K₁ ffERG, N1 K₁, P1 K₁, and N2 K₁ presented an amplitude increase as a function of the light adaptation (N1 K₁ τ value = 2.66 min ± 4.2; P1 K₁ τ value = 2.69 min ± 2.10; and N2 K₁ τ value = 3.49 min ± 2.96). P1 K₁ and N2 K₁ implicit time changed as a function of the light adaptation duration (P1 K₁ τ value = 3.61 min ± 5.2; N2 K₁ τ value = 3.25 min ± 4.8). N1 K₁ had small implicit time changes during the light adaptation. All the K_2,1 components also had nonsignificant changes in amplitude and implicit time during the light adaptation.

Conclusions: Pseudorandom ffERGs showed different mechanisms of adaptation to retinal light. Our results suggest that K₁ ffERG is generated by retinal mechanisms with intermediate- to long-term light adaptation, while K_2.1 ffERG is generated by retinal mechanism with fast light adaptation course.

Keywords: Full-field ERG; Light adaptation; Pseudorandom stimulation; Retina; Visual electrophysiology.