Assembly of Bacterial Genome Sequences from Metagenomes of Spacecraft Assembly Cleanrooms

Velislava Ilieva; Bruce Steel; Jennifer Pratscher; Karen Olsson-Francis; Michael C Macey

doi:10.1128/MRA.01439-20

Assembly of Bacterial Genome Sequences from Metagenomes of Spacecraft Assembly Cleanrooms

Microbiol Resour Announc. 2021 Feb 18;10(7):e01439-20. doi: 10.1128/MRA.01439-20.

Authors

Velislava Ilieva¹, Bruce Steel², Jennifer Pratscher², Karen Olsson-Francis³, Michael C Macey⁴

Affiliations

¹ Faculty of Biology, Medicine and Health, School of Biology Sciences, University of Manchester, Manchester, United Kingdom.
² The Lyell Centre, School of Energy, Geoscience, Infrastructure and Society, Heriot-Watt University, Edinburgh, United Kingdom.
³ AstrobiologyOU, School of Environment, Earth and Ecosystem Sciences, Faculty of Science, Technology, Engineering and Mathematics, The Open University, Milton Keynes, United Kingdom.
⁴ AstrobiologyOU, School of Environment, Earth and Ecosystem Sciences, Faculty of Science, Technology, Engineering and Mathematics, The Open University, Milton Keynes, United Kingdom michael.macey@open.ac.uk.

Abstract

Characterizing the microbiome of spacecraft assembly cleanrooms is important for planetary protection. We report two bacterial metagenome-assembled genomes (MAGs) reconstructed from metagenomes produced from cleanroom samples from the Kennedy Space Center's Payload Hazardous Servicing Facility (KSC-PHSF) during the handling of the Phoenix spacecraft. Characterization of these MAGs will enable identification of the strategies underpinning their survival.