In vivo imaging of a PVD neuron in Caenorhabditis elegans

Xinjian Wang; Tingting Li; Jiawen Hu; Zhigang Feng; Rui Zhong; Wang Nie; Xiaoyan Yang; Yan Zou

doi:10.1016/j.xpro.2021.100309

In vivo imaging of a PVD neuron in Caenorhabditis elegans

STAR Protoc. 2021 Feb 4;2(1):100309. doi: 10.1016/j.xpro.2021.100309. eCollection 2021 Mar 19.

Authors

Xinjian Wang¹, Tingting Li^{1

2}, Jiawen Hu¹, Zhigang Feng¹, Rui Zhong¹, Wang Nie^{1

2}, Xiaoyan Yang^{1

2}, Yan Zou^{1

2}

Affiliations

¹ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
² Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Shanghai 200031, China.

Abstract

The nematode Caenorhabditis elegans nociceptive PVD neurons have highly ordered dendritic branches, making this an ideal model to study the development and organization of dendrites. A ser-2-promoter-driven GFP reporter line wyIs592[ser-2prom-3p::myr-GFP] provides a comprehensive visualization of PVD anatomy. Here, we describe the detailed procedures for imaging a PVD neuron using wyIs592 at late L4 larval stage in vivo by confocal microscopy. This protocol can also be applied to imaging other cells in C. elegans. For complete details on the use and execution of this protocol, please refer to Feng et al. (2020).

Keywords: Microscopy; Model organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Caenorhabditis elegans / growth & development
Caenorhabditis elegans Proteins / physiology
Dendrites / physiology*
Membrane Proteins / physiology
Microscopy, Confocal / methods*
Molecular Imaging / methods*
Neurons / physiology
Nociceptors / physiology
Receptors, Biogenic Amine
Sensory Receptor Cells

Substances

Caenorhabditis elegans Proteins
Membrane Proteins
Receptors, Biogenic Amine
SER-2 protein, C elegans