Dynamics of Membrane Proteins Monitored by Single-Molecule Fluorescence Across Multiple Timescales

Methods Mol Biol. 2020:2168:273-297. doi: 10.1007/978-1-0716-0724-4_13.

Abstract

Single-molecule techniques provide insights into the heterogeneity and dynamics of ensembles and enable the extraction of mechanistic information that is complementary to high-resolution structural techniques. Here, we describe the application of single-molecule Förster resonance energy transfer to study the dynamics of integral membrane protein complexes on timescales spanning sub-milliseconds to minutes (10-9-102 s).

Keywords: FRET; Lipid; Liposome; Protein export; SecA; SecY; Translocation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescence Resonance Energy Transfer / methods*
  • Fluorescence*
  • Humans
  • Membrane Proteins / analysis*
  • Membrane Proteins / chemistry
  • Membrane Proteins / metabolism
  • Protein Conformation
  • Single Molecule Imaging / methods*

Substances

  • Membrane Proteins