Advancing prediction of polycyclic aromatic hydrocarbon bioaccumulation in plants for historically contaminated soils using Lolium multiflorum and simple chemical in-vitro methodologies

Atefeh Esmaeili; Oliver Knox; Albert Juhasz; Susan C Wilson

doi:10.1016/j.scitotenv.2020.144783

Advancing prediction of polycyclic aromatic hydrocarbon bioaccumulation in plants for historically contaminated soils using Lolium multiflorum and simple chemical in-vitro methodologies

Sci Total Environ. 2021 Jun 10:772:144783. doi: 10.1016/j.scitotenv.2020.144783. Epub 2021 Jan 26.

Authors

Atefeh Esmaeili¹, Oliver Knox², Albert Juhasz³, Susan C Wilson²

Affiliations

¹ School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia. Electronic address: atefehesmaeili1985@gmail.com.
² School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia.
³ Future Industries Institute, University of South Australia, Mawson Lakes, SA 5095, Australia.

PMID: 33581513
DOI: 10.1016/j.scitotenv.2020.144783

Abstract

This study compared chemical extraction methods for the prediction of PAH bioaccumulation in ryegrass (Lolium multiflorum) roots in four Manufactured Gas Plant (MGP) historically (>50 years) contaminated soils. The in-vitro methods compared were butanol (BuOH), non-buffered and buffered 2-hydroxypropyl-β-cyclodextrin extractions (HPCD, Buf-HPCD), potassium persulfate oxidation (KPS), solid phase extraction using Tenax resin (Tenax), and polyoxymethylene solid-phase extraction (POM). Extractions were directly compared with bioaccumulation and modelled using equilibrium partitioning theory (EqPT) with a combination of different partitioning parameters (K_OC and K_OW values) that aimed to improve predictions. The PAH accumulation in plant roots showed good correlation with concentrations in soils, and higher concentrations of the 4-6 ring PAHs compared with 2-3 ring PAHs. Plant accumulation of 16 PAHs in L. multiflorum was estimated within a factor of 5 using direct comparison for all bioaccessibility extraction methods. Accumulation values predicted using the calculation approach depended on the combination of K_OC, K_OW parameters and root components (total lipid vs total dry weight) used in calculations. Using K_OC values derived from historically contaminated soils improved accuracy of predicted total root accumulation although precision was low. The combined contribution of PAH in lipid and carbohydrate root components (total dry weight) overestimated accumulation and a lipid only approach using generic partitioning parameters provided more accurate and precise approximation of bioaccumulation in roots of L. multiflorum in the soils. Overall, Tenax, POM and HPCD-based extractions showed promising results for predicting L. multiflorum root accumulation using the different approaches. This work significantly extends current knowledge for integrating simple chemical extractions into ecological risk assessment frameworks for the prediction of plant PAH bioavailability in historically contaminated soils.

Keywords: Aged field soils; Bioaccessibility; Bioassay; K(OC); K(OW); Plant-availability; Root lipids.

MeSH terms

Bioaccumulation
Lolium*
Polycyclic Aromatic Hydrocarbons* / analysis
Soil
Soil Pollutants* / analysis

Substances

Polycyclic Aromatic Hydrocarbons
Soil
Soil Pollutants