Production of a new triterpenoid disaccharide saponin from sequential glycosylation of ganoderic acid A by 2 Bacillus glycosyltransferases

Biosci Biotechnol Biochem. 2021 Feb 24;85(3):687-690. doi: 10.1093/bbb/zbaa055.

Abstract

Ganoderic acid A (GAA) is a lanostane-type triterpenoid, isolated from medicinal fungus Ganoderma lucidum, and possesses multiple bioactivities. In the present study, GAA was sequentially biotransformed by 2 recently discovered Bacillus glycosyltransferases (GT), BtGT_16345 and BsGT110, and the final product was purified and identified as a new compound, GAA-15,26-O-β-diglucoside, which showed 1024-fold aqueous solubility than GAA.

Keywords: ganoderic acid A; glucosides; glycosyltransferase; saponin; triterpenoid.

MeSH terms

  • Bacillus / enzymology*
  • Chromatography, High Pressure Liquid
  • Disaccharides / biosynthesis*
  • Glycosylation
  • Glycosyltransferases / metabolism*
  • Heptanoic Acids / metabolism*
  • Lanosterol / analogs & derivatives*
  • Lanosterol / metabolism
  • Reishi / metabolism
  • Saponins / biosynthesis*
  • Solubility
  • Triterpenes / metabolism*

Substances

  • Disaccharides
  • Heptanoic Acids
  • Saponins
  • Triterpenes
  • Lanosterol
  • ganoderic acid A
  • Glycosyltransferases