In recent years, Brassica juncea has been selected as a special agricultural crop in Jeongseon, Gangwon-do, Korea, and is actively grown there. However, there have been no studies on B. juncea cultivated in Jeongseon (BJJ). Sinigrin, an index component of cruciferous vegetables, has been reported to have antiobesity effects. In this study, we developed a method for obtaining a BJJ extract with optimized sinigrin content, and investigate the antiobesity properties of the BJJ extract and sinigrin. The optimal extraction conditions for BJJ were found to be with 60% ethanol, at 70°C, for 3 hr. Lipid accumulation and ROS production were significantly suppressed in both the BJJ extract and sinigrin-treated groups. Furthermore, BJJ extract and sinigrin were effectively controlled the expression of proteins that regulate lipid accumulation, fatty acid oxidation, and energy metabolism. Thus, BJJ extract containing sinigrin may be used as a health functional food material. PRACTICAL APPLICATIONS: Brassica juncea has been reported to be rich in flavonoids, polyphenols, and glucosinolate, which are secondary vegetable metabolites. In this study, an extraction method to optimize the content of sinigrin in BJJ was established, and the antiobesity mechanism for the extract was confirmed. Lipid accumulation and ROS production were significantly suppressed in both the BJJ extract and sinigrin-treated groups in the study. It was confirmed that the expression of proteins that regulate lipid accumulation, lipid synthesis, fatty acid oxidation, heat generation, and energy metabolism was effectively controlled by the BJJ extract and sinigrin. Therefore, the ethanol extraction method of this study are considered to be useful for the preparation of extracts using cruciferous vegetables, and BJJ extract containing sinigrin have the potential to be used as a health functional food material for obesity.
Keywords: Brassica juncea; antiobesity; optimal extraction condition; sinigrin; western blot.
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