Irisin Protects Against Hind Limb Ischemia Reperfusion Injury

Drug Des Devel Ther. 2021 Feb 4:15:361-368. doi: 10.2147/DDDT.S279318. eCollection 2021.

Abstract

Aim: The aim of this study was to evaluate the effects of irisin in a murine model of hind limb ischemia reperfusion (I/R).

Methods: The mice were divided into four groups (n = 6 in each group): control, irisin, ischemia reperfusion (I/R), and irisin-ischemia reperfusion (I-I/R). Irisin (0.5 µg.g-1, intraperitoneally [i.p.]) was administered 30 min before the I/R procedure. After 2 h of ischemia and 2.5 h of reperfusion, blood and tissue samples were taken for biochemical and histopathological analysis. The results were analyzed by Kruskal-Wallis and Mann-Whitney U-tests.

Results: There was a statistically significant difference in the total antioxidant status (TAS) and total oxidant status (TOS) levels in all the groups. The TAS level in the I/R group was significantly lower than that in the control, irisin, and I-I/R groups, whereas the TOS level was significantly higher in the I/R group as compared with that in the other groups. Caspase-3 activity and caspase-8 activity, indicators of inflammation, were significantly higher in the I/R and I-I/R groups as compared with those in the control and irisin groups.

Conclusion: Irisin may have protective effects in skeletal muscle ischemia reperfusion injury.

Keywords: caspase-3; caspase-8; irisin; ischemia reperfusion; mice.

MeSH terms

  • Animals
  • Dose-Response Relationship, Drug
  • Fibronectins / administration & dosage
  • Fibronectins / metabolism*
  • Hindlimb / drug effects*
  • Hindlimb / metabolism
  • Injections, Intraperitoneal
  • Mice
  • Molecular Structure
  • Protective Agents / administration & dosage
  • Protective Agents / pharmacology*
  • Reperfusion Injury / drug therapy*
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology
  • Structure-Activity Relationship

Substances

  • FNDC5 protein, mouse
  • Fibronectins
  • Protective Agents