Photophysical Properties of Protoporphyrin IX, Pyropheophorbide-a and Photofrin® in Different Conditions

Bauyrzhan Myrzakhmetov; Philippe Arnoux; Serge Mordon; Samir Acherar; Irina Tsoy; Céline Frochot

doi:10.3390/ph14020138

Photophysical Properties of Protoporphyrin IX, Pyropheophorbide-a and Photofrin^® in Different Conditions

Pharmaceuticals (Basel). 2021 Feb 9;14(2):138. doi: 10.3390/ph14020138.

Authors

Bauyrzhan Myrzakhmetov^{1

2}, Philippe Arnoux¹, Serge Mordon³, Samir Acherar⁴, Irina Tsoy², Céline Frochot¹

Affiliations

¹ LRGP UMR 7274, CNRS, University of Lorraine, 54000 Nancy, France.
² Department of Chemistry and Chemical Technology, M.Kh. Dulaty Taraz Regional University, Taraz 080012, Kazakhstan.
³ ONCO-THAI U1189, INSERM, CHU Lille, University of Lille, 59000 Lille, France.
⁴ LCPM UMR 7375, CNRS, University of Lorraine, 54000 Nancy, France.

Abstract

Photodynamic therapy (PDT) is an innovative treatment of malignant or diseased tissues. The effectiveness of PDT depends on light dosimetry, oxygen availability, and properties of the photosensitizer (PS). Depending on the medium, photophysical properties of the PS can change leading to increase or decrease in fluorescence emission and formation of reactive oxygen species (ROS) especially singlet oxygen (¹O₂). In this study, the influence of solvent polarity, viscosity, concentration, temperature, and pH medium on the photophysical properties of protoporphyrin IX, pyropheophorbide-a, and Photofrin^® were investigated by UV-visible absorption, fluorescence emission, singlet oxygen emission, and time-resolved fluorescence spectroscopies.

Keywords: Photofrin®; absorption; fluorescence; photodynamic therapy; protoporphyrin IX; pyropheophorbide-a; singlet oxygen.