Quantification of mtDNA in extracellular vesicles

Beata Soltesz; Balint Nagy

doi:10.1016/bs.mie.2020.07.005

Quantification of mtDNA in extracellular vesicles

Methods Enzymol. 2020:645:119-140. doi: 10.1016/bs.mie.2020.07.005. Epub 2020 Aug 5.

Authors

Beata Soltesz¹, Balint Nagy²

Affiliations

¹ Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary. Electronic address: soltesz.beata@med.unideb.hu.
² Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

PMID: 33565967
DOI: 10.1016/bs.mie.2020.07.005

Abstract

The liquid biopsy preserves a noninvasive technique to analyze promising biomarkers in cell-free bodyfluids, mainly in cell-free plasma. The most cells secrete extracellular vesicles into the extracellular place which can be isolated, analyzed easily due to the wide range of different protocols and commercial kits. The mitochondrial DNA isolated from biofluids can serve as new view in early diagnosis of various diseases (e.g. cancers, cardiovascular diseases). In this chapter, possible protocols of mitochondrial DNA copy number quantification are discussed presenting some ways to determine the mtDNA level of extracellular vesicles in different diseases.

Keywords: Cell-free DNA; Circulating mitochondrial DNA; Exosomes; Extracellular vesicles; Liquid biopsy; mtDNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Mitochondrial / genetics
DNA, Mitochondrial / metabolism
Exosomes* / metabolism
Extracellular Vesicles* / metabolism
Liquid Biopsy
Mitochondria / genetics

Substances

DNA, Mitochondrial