A colorimetric paper-based ATONP-ALP nanobiosensor for selective detection of Cd2+ ions in clams and mussels

Anal Bioanal Chem. 2021 Mar;413(6):1715-1727. doi: 10.1007/s00216-020-03140-3. Epub 2021 Feb 10.

Abstract

A colorimetric paper-based enzyme-coupled antimony tin oxide nanoparticle (ATONP) nanobiosensor for selective detection of Cd2+ ions in clams and mussels is presented. Alkaline phosphatase (ALP) was immobilized on ATONPs via 16-phosphonohexadecanoic acid (16-PHA) to develop ATONP-ALP nanobiosensor. The biosensor was characterized using XPS, Raman spectroscopy, SEM, and EDX. ATONP-ALP nanobiosensor exhibited high selectivity towards detection of Cd2+ ion with a LOD 0.006 μg L-1 and linear range of detection 0.005-1 μg L-1. The developed biosensor was further integrated into a low-cost paper-based format. A visual color change was obtained for Cd2+ ion in the range 0.1-10 μg L-1. The developed biosensor was successfully demonstrated for the analysis of Cd2+ ions in clams with recoveries 101-104%. The ATONP-ALP nanobiosensor was validated using mussel tissue (BCR-668) and the conventional ICP-OES and ICP-MS techniques.

Keywords: Alkaline phosphatase; Antimony tin oxide; Cd2+ ion; Clams; Colorimetric method; Mussels; Paper-based sensor.

MeSH terms

  • Alkaline Phosphatase / chemistry
  • Animals
  • Biosensing Techniques*
  • Bivalvia
  • Cadmium / chemistry*
  • Calibration
  • Colorimetry / methods*
  • Food Analysis / methods
  • Food Contamination / analysis
  • Ions*
  • Kinetics
  • Limit of Detection
  • Microscopy, Electron, Scanning
  • Nanotechnology / methods*
  • Palmitic Acid / chemistry
  • Paper
  • Reproducibility of Results
  • Seafood
  • Spectrum Analysis, Raman

Substances

  • Ions
  • Cadmium
  • Palmitic Acid
  • Alkaline Phosphatase