This study aimed at studying the phycoremediation of petroleum-contaminated water using microalgae biofilm cultured in nutrient-rich water. Microalgae biofilm was grown in a photobioreactor containing water rich in calcium nitrate, manganese chloride, sodium potassium tartrate, calcium phosphate, and ammonium sulfate. Petroleum contaminated water was poured into a photobioreactor, and the substrate containing microalgae biofilm was inserted into the photobioreactor and allowed for eight weeks for biofilm formation. Physicochemical parameters (pH, turbidity, conductivity, sulfate, alkalinity, chloride, TDS, TSS, nitrate, salinity, iron, potassium, phosphate, chlorine, chromium, magnesium, zinc, COD, BOD, and total petroleum hydrocarbon (TPH) of the petroleum contaminated water before and after treatment were determined. The microalgae biofilm used for the treatment was characterized before and after treatment using a Scanning Electron Microscope, X-Ray Fluorescence, and Fourier-transform infrared spectroscopy. The phytochemical constituent of the microalgae biofilm was also determined before and after treatment of the petroleum-contaminated water. The result obtained shows highest removal efficiency of physicochemical parameters (turbidity (81%), conductivity (51.2), sulfate (17.5%), alkalinity 28.4%), chloride (14.6%), TDS (7.9), TSS (26%), nitrate (33%), salinity (23.4), iron (16%), potassium (22%), phosphate (28.2%), chlorine (14%), chromium (13.6%), magnesium (30.3%), zinc (40.5%), COD (8%), BOD (16.7%) and total petroleum hydrocarbon (15%)). The microalgae's characterization shows microalgae biofilm's ability to adsorb pollutants in petroleum-contaminated water due to the presence of microspores and larger surface area of the cells of the microalgae forming the biofilm or due to the absorption efficiency of the extracellular polymeric substances (EPS). The analysis of the microalgae biofilm's phytochemical parameters shows the involvement of the chemicals components in pollutants degradation and antioxidant response of the microalgae to counteract the oxidative effect resulting from the exposure of the microalgae to the contaminated water. NOVELTY STATEMENT This is the first study that attempts the phycoremediation of petroleum contaminated water using microalgae biofilm. The reduction efficiency of the parameters treated in this study is very low compared to that reported in the literature but increases with the retention day. This low reduction efficiency is attributed to the slow assimilation of organic and inorganic pollutants due to the initial growth condition. This study is the first to re-affirm that microalgae biofilm can phycoremediate petroleum-contaminated water by adsorption and assimilation due to the presence of microspores and a larger surface area the cells of the microalgae forming the biofilm or the extracellular polymetric surface covering the biofilm. Several studies have reported that phytochemicals present in microalgae play an antioxidant response role to prevent the microalgae from oxidative damage resulting from water pollution. However, this study is the first to strongly link phytochemicals to the enhancement of pollutants degradation and adsorption by microalgae biofilm.
Keywords: Biofilm; microalgae; oil pollution; oxidative stress; phycoremediation.