Structural changes in cell wall of Japanese radish accompanied by release of rhamnogalacturonan during pressure cooker heating

Abstract

Changes in the cell wall of Japanese radish due to heating at 100 °C or 117 °C for 3 h were examined. Signals in ¹³C cross polarization magic angle spinning solid-state NMR (which detects rigid components) showed no differences between heating temperatures. ¹³C pulse saturation transfer magic angle spinning NMR (which detects flexible components) showed clear temperature-dependent changes in the rhamnose side chains of rhamnogalacturonan. Alcohol-insoluble solids isolated from raw samples were heated in water at 100 °C or 117 °C for 3 h. The concentrations of dissolved sugars and metal ions measured after heating in water at 117 °C were greater than in samples heated at 100 °C, indicating that loosening of cell wall structures increased with temperature, likely via degradation and elution of rhamnogalacturonan followed by β-elimination of homogalacturonan, and fewer interactions between cell wall components, including divalent metal ions. Vegetable shape was retained despite fewer interactions.

Keywords: (13)C CP/MAS NMR; (13)C PST/MAS NMR; Alcohol insoluble solids; Cell wall polysaccharides; Japanese radish; Metalions; Mobility; Rhamnogalacturonan.