Hand-Held Zoom Micro-Imaging System Based on Microfluidic Chip for Point-of-Care Testing (POCT) of Vaginal Inflammation

Ning Yang; Jingxin Peng; Liang Wu; Xue Han; Naila Shaheen; Xiaobo Zou

doi:10.1109/JTEHM.2021.3054556

Hand-Held Zoom Micro-Imaging System Based on Microfluidic Chip for Point-of-Care Testing (POCT) of Vaginal Inflammation

IEEE J Transl Eng Health Med. 2021 Jan 26:9:2800109. doi: 10.1109/JTEHM.2021.3054556. eCollection 2021.

Authors

Ning Yang¹, Jingxin Peng¹, Liang Wu², Xue Han¹, Naila Shaheen¹, Xiaobo Zou³

Affiliations

¹ Department of Electrical and Information EngineeringJiangsu UniversityZhenjiang212013China.
² Department of MedicineJiangsu UniversityZhenjiang212013China.
³ Department of Food and Biological EngineeringJiangsu UniversityZhenjiang212013China.

Abstract

Background: Vaginitis is a common and very private disease, and the current diagnosis is a frequent go to the hospital for testing.

Objective: In order to improve the convenience and speed of detection, in this paper, we have developed a hand-held zoom micro-imaging system based on a microfluidic chip for point-of-care testing (POCT) of vaginal inflammation.

Methods: This system consists of a microfluidic chip, an optical system and a hand-held zoom system assembled with a mobile phone. In terms of hardware, we designed a self-priming microfluidic chip, which can realize automatic sampling and full mixing of samples. We have also developed an optical system that can be adapted to smartphones, which has a lens group with a 37x magnification function and equipped with a zoom system with a focus range of 4mm to 6mm. In terms of software, we proposed an APP that can accurately identify cocci and can determine the inflammation level.

Results: Compared with the recognition rate of the observers in the hospital, the detection accuracy of the portable recognition system is 95%, and after testing the clinical samples, the results were completely consistent with the hospital diagnosis results. The detection limit was 500 CFU / ml, which the relative error was (0.9 ± 0.3) %, and recognition time is 7 seconds.

Conclusion: This system is definitely suitable for women's point-of-care testing (POCT).

Keywords: Bacteria detection; POCT; inflammation assessment; micro-imaging system; microfluidic chip.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Inflammation / diagnosis
Microfluidics*
Point-of-Care Testing
Smartphone
Vaginitis*

Grants and funding

This work was supported in part by the National Key Research and Development Program of China under Grant 2019YFC1606600, in part by the Jiangsu Agricultural Science and Technology Innovation Fund under Grant CX (18) 3043, in part by the Outstanding Youth Science Foundation of Jiangsu Province under Grant BK20180099, in part by the Zhenjiang Key Research and Development Project under Grant NY2019013 and Grant SH2019012, in part by the Project of Agricultural Equipment Department of Jiangsu University under Grant NZXB20200205, and in part by the Jiangsu advantageous Discipline Project (PAPD).