A long-term controlled drug-delivery with anionic beta cyclodextrin complex in layer-by-layer coating for percutaneous implants devices

Beatriz S Verza; Jeroen J J P van den Beucken; João V Brandt; Miguel Jafelicci Junior; Valentim A R Barão; Rodolfo D Piazza; Oya Tagit; Denise M P Spolidorio; Carlos Eduardo Vergani; Erica D de Avila

doi:10.1016/j.carbpol.2020.117604

A long-term controlled drug-delivery with anionic beta cyclodextrin complex in layer-by-layer coating for percutaneous implants devices

Carbohydr Polym. 2021 Apr 1:257:117604. doi: 10.1016/j.carbpol.2020.117604. Epub 2021 Jan 5.

Affiliations

¹ Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, São Paulo State University (UNESP), Humaita, 1680 Araraquara, São Paulo, Brazil. Electronic address: beatriz.verza@hotmail.com.
² Regenerative Biomaterials, Radboudumc, Philips Van Leydenlaan 25, Nijmegen, the Netherlands. Electronic address: jeroen.vandenbeucken@radboudumc.nl.
³ Department of Physical Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo 14801-970, Brazil. Electronic address: joao.brandt@gmail.com.
⁴ Department of Physical Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo 14801-970, Brazil. Electronic address: miguel.jafelicci@unesp.br.
⁵ Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas (UNICAMP), Av. Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil. Electronic address: vbarao@unicamp.br.
⁶ Department of Physical Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo 14801-970, Brazil. Electronic address: rodolfo.piazza@gmail.com.
⁷ Department of Tumor Immunology, Radboudumc and Radboud Institute for Molecular Life Sciences (RIMLS), Geert Grooteplein Zuid, 28 Nijmegen, the Netherlands. Electronic address: oyatagit@gmail.com.
⁸ Department of Physiology and Pathology, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara, São Paulo 14801-903, Brazil. Electronic address: denise.mp.spolidorio@unesp.br.
⁹ Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, São Paulo State University (UNESP), Humaita, 1680 Araraquara, São Paulo, Brazil. Electronic address: carlos.vergani@unesp.br.
¹⁰ Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, São Paulo State University (UNESP), Humaita, 1680 Araraquara, São Paulo, Brazil. Electronic address: erica.fobusp@yahoo.com.br.

PMID: 33541637
DOI: 10.1016/j.carbpol.2020.117604

Abstract

This study demonstrated a drug-delivery system with anionic beta cyclodextrin (β-CD) complexes to retain tetracycline (TC) and control its release from multilayers of poly(acrylic acid) (PAA) and poly(l-lysine) (PLL) in a ten double layers ([PAA/PLL]₁₀) coating onto titanium. The drug-delivery capacity of the multilayer system was proven by controlled drug release over 15 days and sustained released over 30 days. Qualitative images confirmed TC retention within the layer-by-layer (LbL) over 30 days of incubation. Antibacterial activity of TC/anionic β-CD released from the LbL was established against Staphylococcus aureus species. Remarkably, [PAA/PLL]₁₀/TC/anionic β-CD antibacterial effect was sustained even after 30 days of incubation. The non-cytotoxic effect of the multilayer system revealed normal human gingival fibroblast growth. It is expected that this novel approach and the chemical concept to improve drug incorporation into the multilayer system will open up possibilities to make the drug release system more applicable to implantable percutaneous devices.

Keywords: Coating; Cyclodextrin; Drug delivery; Layer-By-Layer; Percutaneous device.

MeSH terms

Acrylic Resins / chemistry
Anions
Anti-Bacterial Agents / administration & dosage
Chitosan / chemistry
Coated Materials, Biocompatible / chemistry*
Delayed-Action Preparations / chemistry*
Drug Delivery Systems*
Drug Liberation
Fibroblasts / drug effects
Humans
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Microbial Sensitivity Tests
Polymers / chemistry
Prostheses and Implants
Prosthesis Design
Staphylococcus aureus / drug effects*
Surface Properties
Tetracycline / chemistry
Titanium / chemistry
beta-Cyclodextrins / chemistry*

Substances

Acrylic Resins
Anions
Anti-Bacterial Agents
Coated Materials, Biocompatible
Delayed-Action Preparations
Polymers
beta-Cyclodextrins
carbopol 940
Chitosan
Titanium
Tetracycline