Plasma membrane H+-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis

Maoxing Zhang; Yin Wang; Xi Chen; Feiyun Xu; Ming Ding; Wenxiu Ye; Yuya Kawai; Yosuke Toda; Yuki Hayashi; Takamasa Suzuki; Houqing Zeng; Liang Xiao; Xin Xiao; Jin Xu; Shiwei Guo; Feng Yan; Qirong Shen; Guohua Xu; Toshinori Kinoshita; Yiyong Zhu

doi:10.1038/s41467-021-20964-4

Plasma membrane H⁺-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis

Nat Commun. 2021 Feb 2;12(1):735. doi: 10.1038/s41467-021-20964-4.

Authors

Maoxing Zhang^#^{1

2}, Yin Wang^#^{2

3}, Xi Chen^#⁴, Feiyun Xu^#¹, Ming Ding¹, Wenxiu Ye^{2

5}, Yuya Kawai⁶, Yosuke Toda^{2

7}, Yuki Hayashi⁶, Takamasa Suzuki⁸, Houqing Zeng⁹, Liang Xiao¹, Xin Xiao¹⁰, Jin Xu¹¹, Shiwei Guo¹, Feng Yan¹², Qirong Shen¹, Guohua Xu¹, Toshinori Kinoshita^{13

14}, Yiyong Zhu¹⁵

Affiliations

¹ Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environment Sciences, Nanjing Agricultural University, Nanjing, China.
² Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Japan.
³ Institute of Ecology, College of Urban and Environmental Sciences and Key Laboratory for Earth Surface Processes of Ministry of Education, Peking University, Beijing, China.
⁴ College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
⁵ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
⁶ Graduate School of Science, Nagoya University, Nagoya, Japan.
⁷ Japan Science and Technology Agency (JST), PRESTO, Kawaguchi, Japan.
⁸ Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi, Japan.
⁹ College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
¹⁰ College of Resources and Environment, Anhui Science and Technology University, Fengyang, China.
¹¹ College of Horticulture, Shanxi Agricultural University, Taigu, China.
¹² Institute of Agronomy and Plant Breeding, Justus Liebig University, Giessen, Germany.
¹³ Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Japan. kinoshita@bio.nagoya-u.ac.jp.
¹⁴ Graduate School of Science, Nagoya University, Nagoya, Japan. kinoshita@bio.nagoya-u.ac.jp.
¹⁵ Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environment Sciences, Nanjing Agricultural University, Nanjing, China. yiyong1973@njau.edu.cn.

^# Contributed equally.

Abstract

Nitrogen (N) and carbon (C) are essential elements for plant growth and crop yield. Thus, improved N and C utilisation contributes to agricultural productivity and reduces the need for fertilisation. In the present study, we find that overexpression of a single rice gene, Oryza sativa plasma membrane (PM) H⁺-ATPase 1 (OSA1), facilitates ammonium absorption and assimilation in roots and enhanced light-induced stomatal opening with higher photosynthesis rate in leaves. As a result, OSA1 overexpression in rice plants causes a 33% increase in grain yield and a 46% increase in N use efficiency overall. As PM H⁺-ATPase is highly conserved in plants, these findings indicate that the manipulation of PM H⁺-ATPase could cooperatively improve N and C utilisation, potentially providing a vital tool for food security and sustainable agriculture.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ammonium Compounds / metabolism
Biological Transport
Cell Membrane / metabolism*
Gene Expression Regulation, Plant / genetics
Gene Expression Regulation, Plant / physiology
Oryza / enzymology*
Oryza / metabolism*
Oryza / physiology
Photosynthesis / genetics
Photosynthesis / physiology
Plant Leaves / enzymology
Plant Leaves / metabolism
Plant Leaves / physiology
Plant Proteins / metabolism*
Plant Roots / enzymology
Plant Roots / metabolism
Plant Roots / physiology
Proton-Translocating ATPases / metabolism*

Substances

Ammonium Compounds
Plant Proteins
Proton-Translocating ATPases