Bio-adhesive Nanoporous Module: Toward Autonomous Gating

Hyuna Jo; Takashi Kitao; Ayumi Kimura; Yoshimitsu Itoh; Takuzo Aida; Kou Okuro

doi:10.1002/anie.202017117

Bio-adhesive Nanoporous Module: Toward Autonomous Gating

Angew Chem Int Ed Engl. 2021 Apr 12;60(16):8932-8937. doi: 10.1002/anie.202017117. Epub 2021 Mar 3.

Authors

Hyuna Jo¹, Takashi Kitao², Ayumi Kimura³, Yoshimitsu Itoh¹, Takuzo Aida^{1

4}, Kou Okuro^{1

5}

Affiliations

¹ Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
² Department of Advanced Materials Science, Graduate School of Frontier Sciences and Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Chiba, 227-8561, Japan.
³ Institute of Engineering Innovation, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo, 113-8656, Japan.
⁴ RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
⁵ Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.

PMID: 33528083
DOI: 10.1002/anie.202017117

Abstract

Here we report a bio-adhesive porous organic module (^Glue COF) composed of hexagonally packed 1D nanopores based on a covalent organic framework. The nanopores are densely decorated with guanidinium ion (Gu⁺ ) pendants capable of forming salt bridges with oxyanionic species. ^Glue COF strongly adheres to biopolymers through multivalent salt-bridging interactions with their ubiquitous oxyanionic species. By taking advantage of its strong bio-adhesive nature, we succeeded in creating a gate that possibly opens the nanopores through a selective interaction with a reporter chemical and releases guest molecules. We chose calmodulin (CaM) as a gating component that can stably entrap a loaded guest, sulforhodamine B (SRB), within the nanopores (^CaM COF⊃SRB). CaM is known to change its conformation on binding with Ca²⁺ ions. We confirmed that mixing ^CaM COF⊃SRB with Ca²⁺ resulted in the release of SRB from the nanopores, whereas the use of weakly binding Mg²⁺ ions resulted in a much slower release of SRB.

Keywords: adhesive materials; gating phenomena; host-guest chemistry; porous materials.

Publication types

Research Support, Non-U.S. Gov't