Synthesis of polyethylene glycol functional bonded silica gel for selective recognition and separation of α-cyclodextrin

Xinying Zheng; Yang Qin; Xiaobing Meng; Zhengyu Jin; Liuping Fan; Jinpeng Wang

doi:10.1016/j.chroma.2021.461917

Synthesis of polyethylene glycol functional bonded silica gel for selective recognition and separation of α-cyclodextrin

J Chromatogr A. 2021 Feb 22:1639:461917. doi: 10.1016/j.chroma.2021.461917. Epub 2021 Jan 20.

Authors

Xinying Zheng¹, Yang Qin¹, Xiaobing Meng¹, Zhengyu Jin¹, Liuping Fan¹, Jinpeng Wang²

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu Province, 214122, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu Province, 214122, China. Electronic address: wangjinpeng1984@126.com.

PMID: 33524932
DOI: 10.1016/j.chroma.2021.461917

Abstract

In this work, we for the first time synthesized the polyethylene glycol (PEG) bonded silica gel via KH-560 as a silane coupling reagent for column chromatography by a solid/liquid surface continuous reaction method. The molecular interaction, structure, morphology, and thermostability was characterized by fourier transform infrared spectroscopy (FTIR), elemental analysis (EA), scanning electronic microscope (SEM) and thermogravimetric analysis (TGA). Given that PEG is capable to self-assemble with α-CD, the PEG bonded silica gel was used as packing of column chromatography to achieve the selective separation of α-cyclodextrin (α-CD) at room temperature and atmospheric pressure. The bonded silica gel column could realize the effective separation of α-CD in the enzymatic hydrolysis mixture, which provides support for industrial separation of α-CD.

Keywords: Alpha-cyclodextrin; Bonded silica gel; Column chromatography; Polyethylene glycol.

MeSH terms

Chromatography, High Pressure Liquid
Hydrolysis
Polyethylene Glycols / chemistry*
Silica Gel / chemical synthesis*
Silicon Dioxide / chemistry
Spectroscopy, Fourier Transform Infrared
Thermogravimetry
alpha-Cyclodextrins / chemistry
alpha-Cyclodextrins / isolation & purification*

Substances

alpha-Cyclodextrins
Polyethylene Glycols
Silica Gel
Silicon Dioxide
alpha-cyclodextrin