Triterpene glycosides and phenylpropane derivatives from Staurogyne concinnula possessing anti-angiogenic activity

Thanh-Hoa Vo; Yu-Chi Lin; Chia-Ching Liaw; Wen-Pin Pan; Jing-Jy Cheng; Ching-Kuo Lee; Yao-Haur Kuo

doi:10.1016/j.phytochem.2021.112666

Triterpene glycosides and phenylpropane derivatives from Staurogyne concinnula possessing anti-angiogenic activity

Phytochemistry. 2021 Apr:184:112666. doi: 10.1016/j.phytochem.2021.112666. Epub 2021 Jan 29.

Authors

Thanh-Hoa Vo¹, Yu-Chi Lin², Chia-Ching Liaw³, Wen-Pin Pan⁴, Jing-Jy Cheng⁵, Ching-Kuo Lee⁶, Yao-Haur Kuo⁷

Affiliations

¹ Ph.D. Program in Clinical Drug Development of Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei, 11031, Taiwan; National Research Institute of Chinese Medicine, Taipei, 11221, Taiwan.
² National Research Institute of Chinese Medicine, Taipei, 11221, Taiwan; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
³ National Research Institute of Chinese Medicine, Taipei, 11221, Taiwan; Department of Biochemical Science and Technology, National Chiayi University, Chiayi, 60004, Taiwan.
⁴ National Research Institute of Chinese Medicine, Taipei, 11221, Taiwan.
⁵ Ph.D. Program in Clinical Drug Development of Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei, 11031, Taiwan; National Research Institute of Chinese Medicine, Taipei, 11221, Taiwan. Electronic address: verona@nricm.edu.tw.
⁶ Ph.D. Program in Clinical Drug Development of Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei, 11031, Taiwan; School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, 11031, Taiwan. Electronic address: cklee@tmu.edu.tw.
⁷ Ph.D. Program in Clinical Drug Development of Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei, 11031, Taiwan; National Research Institute of Chinese Medicine, Taipei, 11221, Taiwan; Graduate Institute of Intergrated Medicine, College of Chinese Medicine, China Medical University, Taichung, 40402, Taiwan. Electronic address: kuoyh@nricm.edu.tw.

PMID: 33524858
DOI: 10.1016/j.phytochem.2021.112666

Abstract

After anti-angiogenic activity screening, the potential n-butanol layer partitioned from the ethanol extract of Staurogyne concinnula was conducted. Further purification by Diaion HP20 column and preparative HPLC chromatography, four undescribed triterpenoid saponin derivatives, along with the known baptisiasaponin I, and four known phenylpropanoid glycosides were isolated and characterized from n-butanol layer. The structures of isolated compounds were elucidated by ESI-MS, 1D, and 2D MNR data. Biological evaluation revealed that baptisiasaponin I possessed significant anti-angiogenic effects (IC₅₀ 4.0 ± 0.2 μM). Further mechanism of action of baptisiasaponin I by inhibition of integrin/FAK/paxillin signaling pathway and its downstream effectors as MMP2 and MMP9 are also presented.

Keywords: Acanthaceae; Anti-angiogenic effect; MMP2; MMP9; Oleanane saponins; Staurogyne concinnula.

MeSH terms

Chromatography, High Pressure Liquid
Glycosides / pharmacology
Molecular Structure
Saponins* / pharmacology
Triterpenes* / pharmacology

Substances

Glycosides
Saponins
Triterpenes