The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the CRISPR/Cas9 system

Tao Dong; Siyao Zhang; Yun Chang; Rui Bai; Youxu Jiang; Shuhong Ma; Ya'nan Li; Hong Feng Jiang; Wen-Jing Lu

doi:10.1016/j.scr.2021.102196

The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the CRISPR/Cas9 system

Stem Cell Res. 2021 Mar:51:102196. doi: 10.1016/j.scr.2021.102196. Epub 2021 Jan 26.

Authors

Tao Dong¹, Siyao Zhang¹, Yun Chang¹, Rui Bai¹, Youxu Jiang¹, Shuhong Ma¹, Ya'nan Li¹, Hong Feng Jiang², Wen-Jing Lu³

Affiliations

¹ Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling-Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China.
² Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling-Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China. Electronic address: jhf@pku.edu.cn.
³ Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling-Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China. Electronic address: wjlu@cellapybio.com.

PMID: 33524674
DOI: 10.1016/j.scr.2021.102196

Abstract

SNTA1 encodes α1-syntrophin, a scaffold protein, which is a component of the dystrophin-associated protein complex. Additionally, α1-syntrophin interacts with SCN5A and nNOS-PMCA4b complex in cardiomyocytes. SNTA1 is a susceptibility locus for arrhythmia and cardiomyopathy. We generated a homozygous SNTA1 knockout human embryonic stem cell (H9SNTA1KO) using the CRISPR/Cas9 system. H9SNTA1KO maintained pluripotency and a normal karyotype and differentiated into three germ layers in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Cell Line
Embryonic Stem Cells
Homozygote
Human Embryonic Stem Cells*
Humans