Protein thiolation index in microvolumes of plasma

Daniela Giustarini; Isabella Dalle-Donne; Graziano Colombo; Aldo Milzani; Annalisa Santucci; Ranieri Rossi

doi:10.1016/j.ab.2021.114125

Protein thiolation index in microvolumes of plasma

Anal Biochem. 2021 Apr 1:618:114125. doi: 10.1016/j.ab.2021.114125. Epub 2021 Jan 30.

Authors

Daniela Giustarini¹, Isabella Dalle-Donne², Graziano Colombo², Aldo Milzani², Annalisa Santucci¹, Ranieri Rossi³

Affiliations

¹ Department of Biotechnology, Chemistry and Pharmacy, (Department of Excellence 2018-2022) University of Siena, Via A. Moro 2, I-53100, Siena, Italy.
² Department of Biosciences, (Department of Excellence 2018-2022) Università Degli Studi di Milano, Via Celoria 26, I-20133, Milan, Italy.
³ Department of Biotechnology, Chemistry and Pharmacy, (Department of Excellence 2018-2022) University of Siena, Via A. Moro 2, I-53100, Siena, Italy. Electronic address: ranieri.rossi@unisi.it.

PMID: 33524411
DOI: 10.1016/j.ab.2021.114125

Abstract

Protein Thiolation Index (PTI) has been recently proposed as a new biomarker of oxidative stress. It is calculated by measuring both free thiols and S-thiolated proteins in plasma with the assumption that this redox ratio is altered by a pro-oxidant stimulus. Here the original protocol was modified and adapted to the use of microvolumes of blood collected by finger prick and down to 3 μl blood was shown to be the lowest volume suitable for this kind of analysis. The new procedure was used to evaluate both the circadian rhythm and the annual fluctuations of PTI in healthy humans.

Keywords: Antioxidant diet; Circadian rhythm; Low molecular mass thiols; Oxidative stress; Protein thiols; S-Thiolation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Blood Proteins / chemistry*
Female
Humans
Male
Middle Aged
Plasma / chemistry*
Sulfhydryl Compounds / chemistry*

Substances

Blood Proteins
Sulfhydryl Compounds