Potent mouse monoclonal antibodies that block SARS-CoV-2 infection

J Biol Chem. 2021 Jan-Jun:296:100346. doi: 10.1016/j.jbc.2021.100346. Epub 2021 Jan 30.

Abstract

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has developed into a global pandemic since its first outbreak in the winter of 2019. An extensive investigation of SARS-CoV-2 is critical for disease control. Various recombinant monoclonal antibodies of human origin that neutralize SARS-CoV-2 infection have been isolated from convalescent patients and will be applied as therapies and prophylaxis. However, the need for dedicated monoclonal antibodies suitable for molecular pathology research is not fully addressed. Here, we produced six mouse anti-SARS-CoV-2 spike monoclonal antibodies that not only exhibit robust performance in immunoassays including western blotting, ELISA, immunofluorescence, and immunoprecipitation, but also demonstrate neutralizing activity against SARS-CoV-2 infection to VeroE6/TMPRSS2 cells. Due to their mouse origin, our monoclonal antibodies are compatible with the experimental immunoassay setups commonly used in basic molecular biology research laboratories, providing a useful tool for future research. Furthermore, in the hope of applying the antibodies of clinical setting, we determined the variable regions of the antibodies and used them to produce recombinant human/mouse chimeric antibodies.

Keywords: SARS-CoV-2; mouse monoclonal antibody; neutralizing antibody; spike.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / isolation & purification
  • Antibodies, Neutralizing / biosynthesis*
  • Antibodies, Neutralizing / chemistry
  • Antibodies, Neutralizing / isolation & purification
  • Antibodies, Viral / biosynthesis*
  • Antibodies, Viral / chemistry
  • Antibodies, Viral / isolation & purification
  • Binding Sites
  • COVID-19 / immunology
  • COVID-19 / prevention & control*
  • COVID-19 / virology
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression
  • Genetic Vectors / chemistry
  • Genetic Vectors / metabolism
  • Humans
  • Mice
  • Neutralization Tests
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • Protein Subunits / administration & dosage
  • Protein Subunits / genetics
  • Protein Subunits / immunology
  • Recombinant Fusion Proteins / administration & dosage
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • SARS-CoV-2 / genetics*
  • SARS-CoV-2 / immunology
  • Spike Glycoprotein, Coronavirus / administration & dosage
  • Spike Glycoprotein, Coronavirus / genetics*
  • Spike Glycoprotein, Coronavirus / immunology
  • Vaccination

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Protein Subunits
  • Recombinant Fusion Proteins
  • Spike Glycoprotein, Coronavirus
  • spike protein, SARS-CoV-2