Catalytic route electrochemiluminescence microscopy of cell membranes with nitrogen-doped carbon dots as nano-coreactants

Chem Commun (Camb). 2021 Feb 28;57(17):2168-2171. doi: 10.1039/d0cc08223f. Epub 2021 Feb 1.

Abstract

Catalytic route electrochemiluminescence (ECL) microscopy enables imaging upper cell membranes with freely diffusing Ru(bpy)32+ as the emitter and nitrogen-doped carbon dots as the nano-coreactants and labels. This strategy provides a vertical resolution when studying the ECL profiles at different heights and realizes the ECL imaging of the externalized phosphatidylserine.

MeSH terms

  • Apoptosis / drug effects
  • Carbon / chemistry*
  • Catalysis
  • Cell Membrane
  • Electrochemistry*
  • HeLa Cells
  • Humans
  • Lipopolysaccharides / toxicity
  • Luminescent Measurements*
  • Microscopy / methods*
  • Nanotechnology / methods
  • Nitrogen / chemistry*
  • Organometallic Compounds / chemistry
  • Quantum Dots / chemistry*
  • Single-Cell Analysis

Substances

  • Lipopolysaccharides
  • Organometallic Compounds
  • Carbon
  • Nitrogen