Transcriptome Profiling of Human Follicle Dermal Papilla Cells in response to Porphyra-334 Treatment by RNA-Seq

Su Yeon Kim; Won Kyong Cho; Hye-In Kim; Seung Hye Paek; Sung Joo Jang; Yeonhwa Jo; Hoseong Choi; Jeong Hun Lee; Sang Hyun Moh

doi:10.1155/2021/6637513

Transcriptome Profiling of Human Follicle Dermal Papilla Cells in response to Porphyra-334 Treatment by RNA-Seq

Evid Based Complement Alternat Med. 2021 Jan 13:2021:6637513. doi: 10.1155/2021/6637513. eCollection 2021.

Authors

Su Yeon Kim^{1

2}, Won Kyong Cho³, Hye-In Kim², Seung Hye Paek², Sung Joo Jang², Yeonhwa Jo³, Hoseong Choi³, Jeong Hun Lee², Sang Hyun Moh²

Affiliations

¹ Department of Bio Cosmetics, Sungkyunkwan University, Suwon 16419, Republic of Korea.
² Anti-aging Research Institute of BIO-FD&C Co., Ltd., Incheon 21990, Republic of Korea.
³ Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.

Abstract

Porphyra-334 is a kind of mycosporine-like amino acid absorbing ultraviolet-A. Here, we characterized porphyra-334 as a potential antiaging agent. An in vitro assay revealed that porphyra-334 dramatically promoted collagen synthesis in fibroblast cells. The effect of porphyra-334 on cell proliferation was dependent on the cell type, and the increase of cell viability by porphyra-334 was the highest in keratinocyte cells among the three tested cell types. An in vivo clinical test with 22 participants demonstrated the possible role of porphyra-334 in the improvement of periorbital wrinkles. RNA-sequencing using human follicle dermal papilla (HFDP) cells upon porphyra-334 treatment identified the upregulation of metallothionein- (MT-) associated genes, confirming the antioxidant role of porphyra-334 with MT. Moreover, the expression of genes involved in nuclear chromosome segregation and the encoding of components of kinetochores was upregulated by porphyra-334 treatment. Furthermore, we found that several genes associated with the hair follicle cycle, the hair follicle structure, the epidermal structure, and stem cells were upregulated by porphyra-334 treatment, suggesting the potential role of porphyra-334 in hair follicle growth and maintenance. In summary, we provided several new pieces of evidence of porphyra-334 as a potential antiaging cosmetic agent and elucidated the expression network in HFDP cells upon porphyra-334.