Endoplasmic Reticulum-Mediated Protein Quality Control and Endoplasmic Reticulum-Associated Degradation Pathway Explain the Reduction of N-glycoprotein Level Under the Lead Stress

Hong Du; Canqi Zheng; Muhmmad Aslam; Xihui Xie; Wanna Wang; Yingquan Yang; Xiaojuan Liu

doi:10.3389/fpls.2020.598552

Endoplasmic Reticulum-Mediated Protein Quality Control and Endoplasmic Reticulum-Associated Degradation Pathway Explain the Reduction of N-glycoprotein Level Under the Lead Stress

Front Plant Sci. 2021 Jan 13:11:598552. doi: 10.3389/fpls.2020.598552. eCollection 2020.

Authors

Hong Du^{1

2}, Canqi Zheng^{1

2}, Muhmmad Aslam^{1

2

3}, Xihui Xie^{1

2}, Wanna Wang^{1

2}, Yingquan Yang^{1

2}, Xiaojuan Liu^{1

2}

Affiliations

¹ Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, College of Sciences, Institute of Marine Sciences, Shantou University, Shantou, China.
² Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China.
³ Faculty of Marine Sciences, Lasbela University of Agriculture, Water & Marine Sciences, Uthal, Pakistan.

Abstract

Different anthropogenic activities result in the continuous increase of metal lead (Pb) in the environment and adversely affect living organisms. Therefore, it is important to investigate the tolerance mechanism in a model organism. Chlamydomonas reinhardtii is an important green eukaryotic model microalga for studying different kinds of biological questions. In this study, the responses of C. reinhardtii were revealed via a comprehensive approach, including physiological, genomic, transcriptomic, glycomic, and bioinformatic techniques. Physiological results showed that the growth rate and soluble protein content were significantly reduced under the high lead stress. Also, the results obtained from the genomic and transcriptomic analyses presented that the endoplasmic reticulum-mediated protein quality control (ERQC) system and endoplasmic reticulum-associated degradation (ERAD) pathway were activated under the third day of high lead stress. The unique upregulated protein disulfide isomerase genes on the ERQC system were proposed to be important for the protein level and protein quality control. The accumulation of specific N-glycans indicated that specific N-glycosylation of proteins might alter the biological functions of proteins to alleviate the Pb stress in alga and/or lead to the degradation of incomplete/misfolded proteins. At the same time, it was observed that genes involved in each process of ERAD were upregulated, suggesting that the ERAD pathway was activated to assist the degradation of incomplete/misfolded proteins. Therefore, it is reasonable to speculate that the reduction of protein level under the high lead stress was related to the activated ERQC system and QRAD pathway. Our findings will provide a solid and reliable foundation and a proposed ERAD working model for further in-depth study of the ERQC system and ERAD pathway under the Pb stress and even other biotic and abiotic stresses.

Keywords: ER-associated degradation pathway; ER-mediated protein quality control; N-glycan; lead; protein level.