Bacillus subtilis-fermented products ameliorate the growth performance and alter cecal microbiota community in broilers under lipopolysaccharide challenge

Abstract

In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge. A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage. The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products. The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age. At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone. The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge. In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group. Principal coordinates analysis indicated distinct clusters between the groups treated with LPS alone and B. subtilis-fermented products in combination with LPS challenge. The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta decreased (P < 0.05) in broilers fed with B. subtilis-fermented products compared with the control group. The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge. Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge. These results demonstrate that B. subtilis-fermented products can improve the growth performance and modulate the gut microflora composition of broilers under immune stress.

Keywords: Bacillus subtilis; broiler; fermented product; lipopolysaccharide; microbiota.