Minor immunosuppressive spiroorthoester group-containing pregnane glycosides from the root barks of Periploca sepium

Bioorg Chem. 2021 Mar:108:104641. doi: 10.1016/j.bioorg.2021.104641. Epub 2021 Jan 12.

Abstract

LC-MS guided chemical investigation of the periploside-rich extract of the root barks of Periploca sepium afforded six new minor pregnane glycosides, named periplosides A1-A6 (1-6). Their structures were characterized on the basis of extensive spectroscopic analysis. Compounds 1-6 were evaluated for their inhibitory activities against the proliferation of T and B lymphocytes in vitro, among them, compound 5 exhibited significant inhibitory activities and the most favorite selective index (SI) values against the proliferation of T lymphocyte (IC50 = 0.30 μM, SI = 176) and B lymphocyte (IC50 = 0.55 μM, SI = 97).

Keywords: Immunosuppression; Periploca sepium; Pregnane glycosides; Spiroorthoester group.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / drug effects*
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Female
  • Glycosides / chemistry
  • Glycosides / isolation & purification
  • Glycosides / pharmacology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Structure
  • Periploca / chemistry*
  • Plant Roots / chemistry*
  • Pregnanes / chemistry
  • Pregnanes / isolation & purification
  • Pregnanes / pharmacology*
  • Structure-Activity Relationship
  • T-Lymphocytes / drug effects*

Substances

  • Glycosides
  • Pregnanes
  • pregnane glycoside