'Laccase-like' properties of coral-like silver citrate micro-structures for the degradation and determination of phenolic pollutants and adrenaline

Abstract

Laccases are multicopper containing oxidase enzymes that are highly important in environmental remediation and biotechnology. To date, complex Copper containing materials have been reported as laccase mimic, and the possibility of a non-Cu laccase mimic remained unknown. In this work, we report an exceptionally simple functional laccase mimic based on coral-like silver citrate (AgCit) microstructures. The AgCit was synthesized by a simple precipitation method and was found to possess excellent laccase-like activity capable of oxidizing phenolic substrates and the endocrine hormone adrenaline. Compared to the natural laccase enzyme, our reported laccase-mimic has a higher υ_max and lower K_m value using adrenaline as a substrate. In addition, the AgCit laccase mimic was observed to be stable at extreme pH, higher temperature, and suitable for long-term storage at room temperature. The laccase-like properties of the AgCit nanozyme were successfully applied for the quantification and degradation of various phenolic pollutants and the adrenaline hormone.

Keywords: Adrenaline sensor; Environmental remediation; Green catalysis; Laccase mimic; Phenolic pollutants.