Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression

Shicheng Su; Shuwen Cao; Jiayu Liu; Xiaoding Xu

doi:10.1016/j.xpro.2020.100275

Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression

STAR Protoc. 2021 Jan 15;2(1):100275. doi: 10.1016/j.xpro.2020.100275. eCollection 2021 Mar 19.

Authors

Shicheng Su^{1

2}, Shuwen Cao^{1

2}, Jiayu Liu^{1

2}, Xiaoding Xu^{1

2}

Affiliations

¹ Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.
² Medical Research Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.

Abstract

Targeted regulation of mitochondrial gene expression is challenging due to the lack of a mitochondria-specific delivery system. We have previously developed various stimuli-responsive nanoparticle (NP)-based delivery systems to transport nucleic acids for regulation of target gene expression. This protocol describes the design and preparation of an NP platform for mitochondria-specific gene delivery (mito-NP). We use mito-NP in primary liver fibroblasts that are transplanted into mice. Mito-NP can be used to deliver various nucleic acid therapeutics and to treat mitochondria-regulated diseases. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).

Keywords: Gene Expression; Genetics; Molecular Biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fibroblasts* / metabolism
Fibroblasts* / transplantation
Gene Expression Regulation*
Gene Transfer Techniques*
Humans
Liver / metabolism*
Mice
Mitochondria, Liver* / genetics
Mitochondria, Liver* / metabolism