Influence of single nucleotide polymorphisms among cigarette smoking and non-smoking patients with coronary artery disease, urinary bladder cancer and lung cancer

Nongnit Laytragoon Lewin; Jan-Erik Karlsson; David Robinsson; Matida Fagerberg; Magnus Kentsson; Shariel Sayardoust; Mats Nilsson; Levar Shamoun; Bengt-Åke Andersson; Sture Löfgren; Lars Erik Rutqvist; Freddi Lewin

doi:10.1371/journal.pone.0243084

Influence of single nucleotide polymorphisms among cigarette smoking and non-smoking patients with coronary artery disease, urinary bladder cancer and lung cancer

PLoS One. 2021 Jan 28;16(1):e0243084. doi: 10.1371/journal.pone.0243084. eCollection 2021.

Authors

Affiliations

¹ Dept Laboratory Medicine, Ryhov Hospital, Jönköping, Sweden.
² Dept of Internal Medicine, Ryhov Hospital, Jönköping, Sweden.
³ Dept of Health Medicine and Caring Sciences, Linköping University, Linköping, Sweden.
⁴ Dept Urology, Ryhov Hospital, Jönköping, Sweden.
⁵ Dept Periodontology, Ryhov Hospital, Jönköping, Sweden.
⁶ Futurum, Academy of Health and Care, Region Jönköping, Jönköping, Sweden.
⁷ Dept Medical and Health Sciences, Linköping University, Linköping, Sweden.
⁸ Scientific Affairs Group, Swedish Match AB, Stockholm, Sweden.
⁹ Dept Oncology, Ryhov Hospital, Jönköping, Sweden.

Abstract

Introduction: Cigarette smoke is suggested to be a risk factor for coronary artery disease (CAD), urinary bladder cancer (UBCa) or lung cancer (LCa). However, not all heavy smokers develop these diseases and elevated cancer risk among first-degree relatives suggests an important role of genetic factor.

Methods: Three hundred and ten healthy blood donors (controls), 98 CAD, 74 UBCa and 38 LCa patients were included in this pilot study. The influence of 92 single nucleotide polymorphisms (SNPs) and impact of cigarette smoking were analysed.

Results: Out of 92 SNPs tested, differences in distribution of 14 SNPs were detected between controls and patient groups. Only CTLA4 rs3087243 showed difference in both CAD and UBCa patient group compared to control group. Stratified by smoking status, the impact of smoking was associated to frequencies of 8, 3 and 4 SNPs in CAD, UBCa, LCa patients, respectively. None of these 92 SNPs showed a statistically significant difference to more than one type of disease among smoking patients. In non-smoking patients, 7, 3 and 6 SNPs were associated to CAD, UBCa, LCa, respectively. Out of these 92 SNPs, CTLA4 rs3087243 was associated to both non-smoking CAD and UBCa. The XRCC1 rs25487 was associated to both non-smoking UBCa and LCa.

Conclusion: SNPs might be important risk factors for CAD, UBCa and LCa. Distribution of the SNPs was specific for each patient group, not a random event. Impact of cigarette smoking on the disease was associated to the specific SNP sequences. Thus, smoking individuals with SNPs associated to risk of these serious diseases is an important target group for smoking cessation programs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Case-Control Studies
Cigarette Smoking / genetics*
Coronary Artery Disease / genetics*
Female
Genetic Predisposition to Disease*
Humans
Lung Neoplasms / genetics*
Male
Middle Aged
Polymorphism, Single Nucleotide / genetics*
Urinary Bladder Neoplasms / genetics*

Grants and funding

Grants were received from Jönköping Clinical Cancer Research Foundation (Grant 110426-1), Futurum the Academy for health and care (Grant 144631), Medical Research Council of Southeast Sweden (FORSS, Grant 567001), Internal research Laboratory Medicine (Grant and Swedish Match AB. The funders had no role in the study design, data collection, analysis, decision to publish or the preparation of the manuscript.