Protein detection based on rolling circle amplification sensors

Haixia Shi; Jingjie Cui; Husseini Sulemana; Wunian Wang; Li Gao

doi:10.1002/bio.4017

Protein detection based on rolling circle amplification sensors

Luminescence. 2021 Jun;36(4):842-848. doi: 10.1002/bio.4017. Epub 2021 Feb 7.

Authors

Haixia Shi¹, Jingjie Cui², Husseini Sulemana³, Wunian Wang¹, Li Gao⁴

Affiliations

¹ P. E. Department of Jiangsu University, Zhenjiang, China.
² School of Automation, Hangzhou Dianzi University, Hangzhou, China.
³ School of the Environment and Safety Engineering, Zhenjiang, China.
⁴ Institute of Life Sciences, Jiangsu University, Zhenjiang, China.

PMID: 33502072
DOI: 10.1002/bio.4017

Abstract

Rolling circle amplification (RCA) is an isothermal process under the action of DNA polymerases. Large-scale DNA templates have been generated using RCA for target detection. Some signal amplification strategies including optical sensors and electrochemical sensors based on RCA have been applied to achieve sensitive detection. Sensors based on RCA have attracted increasing interest. Advances in RCA-based sensors for protein detection are reviewed in this paper. The advantages and detection mechanisms of sensors based on RCA are revealed and discussed. Finally, possible challenges and future perspectives are also outlined.

Keywords: RCA; protein detection; sensors.

Publication types

Review

MeSH terms

Biosensing Techniques*
Nucleic Acid Amplification Techniques*

Abstract

Publication types

MeSH terms

Grants and funding