Next-generation sequencing yields the complete chloroplast genome of C. goeringii acc. smg222 and phylogenetic analysis

Heng Wang; So-Yeon Park; Ah-Rim Lee; Seong-Gyu Jang; Da-Eun Im; Tae-Hwan Jun; Joohyun Lee; Jong-Wook Chung; Tae-Ho Ham; Soon-Wook Kwon

doi:10.1080/23802359.2018.1437812

Next-generation sequencing yields the complete chloroplast genome of C. goeringii acc. smg222 and phylogenetic analysis

Mitochondrial DNA B Resour. 2018 Feb 12;3(1):215-216. doi: 10.1080/23802359.2018.1437812.

Authors

Affiliations

¹ Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang, Republic of Korea.
² Department of Applied Bioscience, Konkuk University, Seoul, Republic of Korea.
³ Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, Republic of Korea.
⁴ Department of Agricultural Science, Korea National Open University, Seoul, Republic of Korea.

Abstract

Spring orchid (Cymbidium goeringii) is one of the most important species belonging to Orchidaceae owing to its aesthetic appeal, fragrant flowers and ideal characteristics for using as a houseplant. In this study, the complete chloroplast genome of Korean C. goeringii acc. smg222 was determined by Illumina sequencing. The circular double-stranded DNA of 148,441 bp consisted of two inverted repeat regions of 25,610 bp each, a large single copy region of 83,311 bp, and a small single copy region of 13,910 bp. The genome contained 122 genes, of which 104 were unique and 18 were duplicated within the IRs. The 104 unique genes included 70 protein-coding genes, 30 distinct tRNA genes, and four rRNA genes. Phylogenetic tree analysis revealed that C. goeringii acc. smg222 was clustered with Cymbidium kanran, a cymbidium species native to Korea.

Keywords: Cymbidium goeringii acc. smg222; Illumina sequencing; chloroplast genome.