Sucrose non-fermenting-1-related protein kinase 2 s (SnRK2 s) are important stress-related plant protein kinases in plants. The interaction partners and phosphorylation substrates of group II and III SnRK2 s in Arabidopsis thaliana have been identified, but similar data for group I SnRK2 s are very limited. Here, we used a yeast two-hybrid (Y2H) screen to find proteins that interact with Arabidopsis AtSnRK2.4, a group I SnRK2. The transcription factor AtMYB21 was identified as an AtSnRK2.4 interaction partner, and its interaction with AtSnRK2.4 was confirmed by an in vitro pull-down assay and a bimolecular fluorescence complementation (BiFC) assay. A subcellular localization assay demonstrated that AtSnRK2.4 and AtMYB21 were located in the cytoplasm and nucleus of onion epidermal cells. AtSnRK2.4 and AtMYB21 were expressed in many tissues and upregulated in response to NaCl stress. Transgenic plants that overexpressed AtSnRK2.4 or AtMYB21 gene exhibited enhanced tolerance to salt stress at germination and post-germination stages. Moreover, the expression of downstream stress-responsive genes was upregulated in salt-stressed AtSnRK2.4 and AtMYB21 transgenic Arabidopsis. These results suggest that AtSnRK2.4 may act synergistically with AtMYB21 to mediate the response to salt stress through the upregulation of downstream stress-responsive genes.
Keywords: Arabidopsis; AtMYB21; AtSnRK2.4; Salt stress.
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