Biotransformation of doxycycline by Brevundimonas naejangsanensis and Sphingobacterium mizutaii strains

Ting He; Jianguo Bao; Yifei Leng; Daniel Snow; Shuqiong Kong; Tong Wang; Xu Li

doi:10.1016/j.jhazmat.2021.125126

Biotransformation of doxycycline by Brevundimonas naejangsanensis and Sphingobacterium mizutaii strains

J Hazard Mater. 2021 Jun 5:411:125126. doi: 10.1016/j.jhazmat.2021.125126. Epub 2021 Jan 13.

Authors

Ting He¹, Jianguo Bao², Yifei Leng³, Daniel Snow⁴, Shuqiong Kong⁵, Tong Wang⁵, Xu Li⁶

Affiliations

¹ School of Environmental Studies, China University of Geosciences, No. 388 Lumo Road, Wuhan, Hubei 430074, China; Department of Civil and Environmental Engineering, University of Nebraska-Lincoln, 900 N 16th St., W150D Nebraska Hall, Lincoln, NE 68588-0531, USA.
² School of Environmental Studies, China University of Geosciences, No. 388 Lumo Road, Wuhan, Hubei 430074, China. Electronic address: bjianguo@cug.edu.cn.
³ School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068, China.
⁴ Water Sciences Laboratory, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
⁵ School of Environmental Studies, China University of Geosciences, No. 388 Lumo Road, Wuhan, Hubei 430074, China.
⁶ Department of Civil and Environmental Engineering, University of Nebraska-Lincoln, 900 N 16th St., W150D Nebraska Hall, Lincoln, NE 68588-0531, USA. Electronic address: xuli@unl.edu.

PMID: 33486232
DOI: 10.1016/j.jhazmat.2021.125126

Abstract

The fate of doxycycline (DC), a second generation tetracycline antibiotic, in the environment has drawn increasing attention in recent years due to its wide usage. Little is known about the biodegradability of DC in the environment. The objective of this study was to characterize the biotransformation of DC by pure bacterial strains with respect to reaction kinetics under different environmental conditions and biotransformation products. Two bacterial strains, Brevundimonas naejangsanensis DD1 and Sphingobacterium mizutaii DD2, were isolated from chicken litter and characterized for their biotransformation capability of DC. Results show both strains rely on cometabolism to biotransform DC with tryptone as primary growth substrate. DD2 had higher biotransformation kinetics than DD1. The two strains prefer similar pHs (7 and 8) and temperature (30 °C), however, they exhibited opposite responses to increasing background tryptone concentration. While hydrolysis converted DC to its isomer or epimer, the two bacterial strains converted DC to various biotransformation products through a series of demethylation, dehydration, decarbonylation and deamination. Findings from the study can be used to better predict the fate of DC in the environment.

Keywords: Biotransformation; Doxycycline; Kinetic; Transformation products.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotransformation
Caulobacteraceae
Doxycycline*
Sphingobacterium*

Substances

Doxycycline

Supplementary concepts

Brevundimonas naejangsanensis
Sphingobacterium mizutaii