Rapid detection of SARS-CoV-2, replicating or non-replicating, using RT-PCR

Ming Liao; Jianmin Wu; Manman Dai; Huanan Li; Nan Yan; Runyu Yuan; Chungen Pan

doi:10.1016/j.ijid.2021.01.043

Rapid detection of SARS-CoV-2, replicating or non-replicating, using RT-PCR

Int J Infect Dis. 2021 Mar:104:471-473. doi: 10.1016/j.ijid.2021.01.043. Epub 2021 Jan 21.

Authors

Ming Liao¹, Jianmin Wu², Manman Dai³, Huanan Li³, Nan Yan³, Runyu Yuan⁴, Chungen Pan⁵

Affiliations

¹ Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. Electronic address: mliao@scau.edu.cn.
² Haid Research Institute, Guangdong HaidGroup Co., Ltd., Guangzhou, China.
³ College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
⁴ Guangdong Provincial Institute of Public Health, Guangzhou, China.
⁵ Haid Research Institute, Guangdong HaidGroup Co., Ltd., Guangzhou, China. Electronic address: chungenp@haid.com.cn.

Abstract

To identify animals susceptible to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection or to determine whether SARS-CoV-2 contaminated meat is from a SARS-CoV-2-infected animal, a convenient and safe method was developed for rapid detection of SARS-CoV-2 in a replicating or non-replicating status in samples using reverse transcriptase-polymerase chain reaction (RT-PCR). This strategy can also be applied to develop assays for the detection of other viruses, either replicating or not.

Keywords: Negative-sense RNA; RT-PCR; SARS-CoV-2.

MeSH terms

Animals
COVID-19 / diagnosis*
COVID-19 Testing / methods*
Humans
RNA, Viral / analysis*
Reverse Transcriptase Polymerase Chain Reaction*
SARS-CoV-2 / physiology*
Sensitivity and Specificity
Virus Replication*

Substances

RNA, Viral